Random chimeragenesis on transient templates

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Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate.[1] For example, RACHITT can be used to generate increased rate and extent of biodesulfurization of diesel by modification of dibenzothiophene mono-oxygenase. DNA shuffling is a similar but less powerful method used in directed evolution experiments.

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  1. ↑ Script error: No such module "Citation/CS1".
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