PIPES

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PIPES
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Template:Longitem C8H18N2O6S2
Molar mass 302.37
Appearance White powder
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PIPES (piperazine-N,NTemplate:Prime-bis(2-ethanesulfonic acid)) is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]

Applications

PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.[5]

See also

References

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  2. Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
  3. Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
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