Levansucrase
Template:Short description Template:Infobox enzyme Levansucrase (EC 2.4.1.10) is an enzyme that catalyzes the chemical reaction
- sucrose + (2,6-beta-D-fructosyl)n glucose + (2,6-beta-D-fructosyl)n+1
Thus, the two substrates of this enzyme are sucrose and (2,6-beta-D-fructosyl)n, whereas its two products are glucose and (2,6-beta-D-fructosyl)n+1.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is sucrose:2,6-beta-D-fructan 6-beta-D-fructosyltransferase. Other names in common use include sucrose 6-fructosyltransferase, beta-2,6-fructosyltransferase, and beta-2,6-fructan:D-glucose 1-fructosyltransferase. This enzyme participates in starch and sucrose metabolism and two-component system - general.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1OYG, 1PT2, and 1W18.
References
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SacB counter-selection relies on the toxic product produced by the SacB gene. sacB comes from the gram-positive bacteria Bacillus subtilis and encodes the enzyme levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose medium will select for cells that contain constructs that have lost the sacB gene.[1]
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