Janus Green B

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Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[1]

The indicator Janus Green B changes colour according to the amount of oxygen present.[2] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.

References

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  1. Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
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