Carnoy's solution

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Template:Short description Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid, 1 gram of ferric chloride.[1][2]

Carnoy's solution is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).[3][4][5]

The invention of Carnoy's solution is attributed to Jean-Baptiste Carnoy, a pioneering 19th century cytologist.[6]

Uses

Some of the uses of Carnoy's solution are:

  • Enhancing lymph node detection during dissection of cadavers.[7]
  • Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus.[8]
  • Applied directly following enucleation for the treatment of odontogenic keratocysts.[9][10][11]
  • Direct application following enucleation (Cuba) for certain kinds of unicystic ameloblastomas.[12] This appears to decrease the likelihood of recurrence over enucleation alone.[13] Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.[14]
  • As a fixative for pap smear samples.[15]
  • As a fixative agent for both nuclear and mitochondrial DNA in various tissues.[16]
  • As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acid-Schiff base.[17]

References

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