Calf-intestinal alkaline phosphatase
Template:Infobox nonhuman protein Template:Enzyme
Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate.[3] This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.
Applications
Calf-intestinal alkaline phosphatase can serve as an effective tool for removing uranium from groundwater and soil that can pose major health risks.[4] Furthermore, the toxicity of lipopolysaccharide (LPS) was mitigated by calf-intestinal alkaline phosphatase in mice and piglets, which indicates that it could be a promising new therapeutic agent for treating diseases associated with LPS.[5]
References
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