CHES (buffer)

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CHES
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MeSH 2-(N-cyclohexylamino)ethanesulfonic+acid
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CHES (N-cyclohexyl-2-aminoethanesulfonic acid) is a buffering agent. CHES buffers have a useful range of pH 8.6–10.[1]

It typically appears as a white crystalline powder.

Effect of impurities

Commercial prep of CHES (and other sulfonylethyl buffers like MES, BES, and PIPES) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (pM) inhibitor of RNA binding proteins and enzymes.[2]

References

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External links

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