Bisbenzimide
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Bisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications.[1] Several related chemical compounds are used for similar purposes and are collectively called Hoechst stains.
Application
Bisbenzimide tends to bind to adenine–thymine-rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.
References
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External links
- Fluorescence Spectra: http://www.fluorophores.tugraz.at/substance/463