View source for RNA-3'-phosphate cyclase

{{infobox enzyme
| Name       = RNA-3′-phosphate cyclase
| EC_number  = 6.5.1.4
| CAS_number = 85638-41-1
| GO_code    = 0003963
| image      =
| width      = 
| caption    =  
}}
In [[enzymology]], a '''RNA-3′-phosphate cyclase''' ({{EnzExplorer|6.5.1.4}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:ATP + RNA 3'-terminal-phosphate <math>\rightleftharpoons</math> AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate

Thus, the two [[substrate (biochemistry)|substrates]] of this enzyme are [[adenosine triphosphate|ATP]] and [[RNA 3'-terminal-phosphate]], whereas its 3 [[product (chemistry)|products]] are [[adenosine monophosphate|AMP]], [[diphosphate]], and [[RNA terminal-2',3'-cyclic-phosphate]].

This enzyme belongs to the family of [[ligase]]s, specifically those forming phosphoric-ester bonds.  The [[List of enzymes|systematic name]] of this enzyme class is '''RNA-3'-phosphate:RNA ligase (cyclizing, AMP-forming)'''. This enzyme is also called '''RNA cyclase'''.

==Structural studies==

As of 2010, three [[tertiary structure|structures]] have been solved for this class of enzymes, with [[Protein Data Bank|PDB]] accession codes {{PDB2|1QMH}} and {{PDB2|1QMI}}, (un-[[adenylation|adenylated]])<ref name="pmid10673421">{{cite journal | vauthors = Palm GJ, Billy E, Filipowicz W, Wlodawer A | title = Crystal structure of RNA 3'-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology | journal = Structure | volume = 8 | issue = 1 | pages = 13–23 |date=January 2000 | pmid = 10673421 | doi = 10.1016/S0969-2126(00)00076-9| doi-access = free }}</ref> and {{PDB2|3KGD}} (adenylated).<ref name="pmid20399182">{{cite journal | vauthors = Tanaka N, Smith P, Shuman S | title = Structure of the RNA 3'-phosphate cyclase–adenylate intermediate illuminates nucleotide specificity and covalent nucleotidyl transfer | journal = Structure | volume = 18 | issue = 4 | pages = 449–57 |date=March 2010 | pmid = 20399182 | pmc = 2858066 | doi = 10.1016/j.str.2010.01.016 }}</ref>

==References==
{{reflist}}

== Further reading ==
{{refbegin}}
* {{cite journal | vauthors = Filipowicz W, Konarska M, Gross HJ, Shatkin AJ | date = 1983 | title = RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract | journal = Nucleic Acids Res.  | volume = 11 | pages = 1405&ndash;18  | pmid = 6828385 | doi = 10.1093/nar/11.5.1405 | issue = 5 | pmc = 325805 }}
* {{cite journal | vauthors = Reinberg D, Arenas J, Hurwitz J | date = 1985 | title = The enzymatic conversion of 3'-phosphate terminated RNA chains to 2',3'-cyclic phosphate derivatives | journal = J. Biol. Chem.  | volume = 260 | pages = 6088&ndash;97  | pmid = 2581947 | issue = 10 | doi = 10.1016/S0021-9258(18)88941-4 | doi-access = free }}
{{refend}}

{{Phosphoric ester and nitrogen-metal ligases}}
{{Enzymes}}
{{Portal bar|Biology|border=no}}

<ref>{{Cite journal|pmid = 28132487|year = 2016|last1 = Filipowicz|first1 = W.|title = RNA 3'-terminal phosphate cyclases and cyclase-like proteins|journal = Postepy Biochemii|volume = 62|issue = 3|pages = 327–334| doi=10.18388/pb.2016_32 | s2cid=36153563 |doi-access = free}}</ref>
<ref>{{Cite journal|doi=10.1016/S0969-2126(00)00076-9|title=Crystal structure of RNA 3′-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology|year=2000|last1=Palm|first1=Gottfried J.|last2=Billy|first2=Eric|last3=Filipowicz|first3=Witold|last4=Wlodawer|first4=Alexander|journal=Structure|volume=8|issue=1|pages=13–23|pmid=10673421|doi-access=free}}</ref>

[[Category:EC 6.5.1]]
[[Category:Enzymes of known structure]]


{{ligase-stub}}