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- ...e lane labelled "control" is for quality control purposes and contains the DNA fragment but not treated with DNase I.]] ...n enzyme, [[deoxyribonuclease]] (DNase, for short), to cut the end-labeled DNA radioactively, followed by [[gel electrophoresis]] to detect the resulting ...3 KB (428 words) - 10:38, 28 February 2025
- ...irst14=Chongjun|last15=Little|first15=Deane|title=Chip Technology |chapter=Sequencing by Hybridization (SBH): Advantages, Achievements, and Opportunities|series= ...ay-authors=et al|title=Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase ...3 KB (414 words) - 06:14, 30 July 2022
- {{Short description|Punctual group of DNA}} '''Polony''' is a contraction of "polymerase colony," a small colony of [[DNA]]. ...2 KB (334 words) - 17:15, 8 June 2024
- {{short description|DNA sequencing method}} ...technique, also known as "directed sequencing," employs a series of Sanger sequencing reactions to either confirm the reference sequence of a known plasmid or PC ...9 KB (1,316 words) - 21:29, 11 July 2023
- ...of overlapping DNA segments that together represent a consensus region of DNA}} ...egments that together represent a [[Consensus sequence|consensus region of DNA]].<ref name="contig assembly">Gregory, S. ''Contig Assembly''. Encyclopedi ...10 KB (1,440 words) - 12:45, 26 March 2025
- {{Short description|Method of DNA sequencing}} ...he sequencing is performed by detecting the nucleotide incorporated by a [[DNA polymerase]]. Pyrosequencing relies on light detection based on a chain rea ...9 KB (1,217 words) - 22:28, 25 May 2025
- ...stranded DNA) molecules which act as surface receptors. When complementary DNA strands hybridize to the receptors, the charge distribution near the surfac ...for [[DNA sequencing]]. Their main advantage compared to optical detection methods in common use today is that they do not require labeling of molecules. Furt ...2 KB (283 words) - 21:37, 30 August 2022
- Genetic analysis can be used generally to describe methods both used in and resulting from the sciences of genetics and molecular biol ...r gel electrophoresis analysis.jpg|thumb|right|FDA microbiologist prepares DNA samples for gel electrophoresis analysis]] ...8 KB (1,237 words) - 09:59, 20 February 2025
- ...n made in yeast (Pelechano et al., 2009). A general correspondence of both methods has been detected but GRO is more sensitive and quantitative. It has to be ...first2 = J | last3 = Lis | first3 = JT | year = 2008 | title = Nascent RNA Sequencing Reveals Widespread Pausing and Divergent Initiation at Human Promoters | jo ...5 KB (747 words) - 13:46, 10 March 2025
- ...ted on a polyacrylamide gel and detected by autoradiography. Typically, a sequencing ladder using the same primer is also on the gel, allowing for rapid identif ...e (e.g. [[Sanger sequencing]]), preferably by using the same primer on the DNA template strand. The exact nucleotide by which the transcription starts at ...3 KB (494 words) - 11:26, 4 August 2021
- {{about|the genetics definition of "sequencing"|the sense of "sequencing" used in electronic music|music sequencer|sequence learning in cognitive sc ...incorrectly called the primary sequence) of an unbranched [[biopolymer]]. Sequencing results in a symbolic linear depiction known as a '''sequence''' which succ ...13 KB (1,878 words) - 22:46, 2 August 2025
- {{Short description|Chain-elongating inhibitor of DNA polymerase}} ...[[DNA polymerase]], used in the [[Sanger sequencing|Sanger method for DNA sequencing]].<ref name = MeisRaghavachari /> They are also known as 2',3' because bot ...10 KB (1,315 words) - 03:24, 27 January 2025
- {{short description|Deliberate fragmentation of DNA in preparation for analysis}} ...h this term is used for other procedures as well. In a restriction digest, DNA molecules are cleaved at specific regions of 4-12 [[nucleotide]]s in length ...6 KB (890 words) - 05:51, 9 June 2025
- ...expanding due to the dramatic decrease in the cost of [[genome sequencing|sequencing genomes]]. The study of stem cell genomics has wide reaching implications i ...ization of the pluripotent stem cell through [[DNA]] and [[transcriptome]] sequencing and looking at the epigenetic changes of stem cells and subsequent products ...5 KB (745 words) - 08:13, 9 February 2024
- {{Short description|Type of DNA library}} ...rons]], and other regulatory elements found in a [[genomic library|genomic DNA library]].<ref>{{Cite web |title=cDNA Libraries - an overview {{!}} Science ...8 KB (1,166 words) - 10:44, 30 June 2025
- {{Short description|DNA construct}} ...a [[p1-derived artificial chromosome|PAC]] has also been produced from the DNA of P1 bacteriophage. ...8 KB (1,096 words) - 08:17, 21 July 2025
- {{short description|Lab technique for separation of DNA}} ...<ref name="Kaufmann1998" /> This allows for the direct analysis of genomic DNA.<ref name="HerschlebAnaniev2007" /> ...7 KB (850 words) - 19:42, 26 May 2025
- ...s is useful in [[genetics]] and [[biology]] research, [[forensics]], and [[DNA fingerprinting]]. ...om (or added to) the VNTR via [[Genetic recombination|recombination]] or [[DNA replication|replication]] errors, leading to alleles with different numbers ...8 KB (1,218 words) - 23:20, 3 April 2025
- ...gham RP, Tainer JA | title = Structure and function of the multifunctional DNA-repair enzyme exonuclease III | journal = Nature | volume = 374 | issue = 6 ...t, resulting in coordinated progressive deletions within the population of DNA [[molecule]]s.<ref name="Image source"/> ...5 KB (642 words) - 01:31, 22 June 2024
- {{short description|In genetics, a stretch of DNA sequences located between genes}} ...2008}}</ref> Intergenic regions may contain functional elements and [[junk DNA]]. ...7 KB (883 words) - 14:06, 14 September 2024