http://debianws.lexgopc.com/wiki143/index.php?action=history&feed=atom&title=SSC_buffer 2026-05-08T22:30:35Z Revision history for this page on the wiki MediaWiki 1.43.1 http://debianws.lexgopc.com/wiki143/index.php?title=SSC_buffer&diff=6945860&oldid=prev 2022-01-16T22:18:18Z

<p>Moving <a href="/wiki143/index.php?title=Category:Buffers&amp;action=edit&amp;redlink=1" class="new" title="Category:Buffers (page does not exist)">Category:Buffers</a> to <a href="/wiki143/index.php?title=Category:Buffer_solutions" title="Category:Buffer solutions">Category:Buffer solutions</a> per <a href="https://en.wikipedia.org/wiki/Categories_for_discussion/Speedy" class="extiw" title="wikipedia:Categories for discussion/Speedy">Wikipedia:Categories for discussion/Speedy</a></p> <p><b>New page</b></p><div>In [[biochemistry]] and [[molecular biology]], &#039;&#039;&#039;saline-sodium citrate (SSC) buffer&#039;&#039;&#039; is used as a hybridization [[buffer (chemistry)|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[in situ hybridization]], [[DNA Microarray]] or [[Northern blotting]]. 20X SSC may be used to prevent drying of [[agarose gel]]s during a vacuum transfer. <br /> <br /> A 20X stock solution consists of 3 [[molar (concentration)|M]] [[sodium chloride]] and 300 [[milli-|m]]M [[trisodium citrate]] (adjusted to [[pH]] 7.0 with [[hydrochloric acid|HCl]]).<br /> <br /> ==See also==<br /> *[[Southern Blot]]<br /> *[[Northern Blot]]<br /> *[[DNA Microarray]]<br /> *[[DNA Fingerprinting]]<br /> <br /> ==References==<br /> * [http://www.cellgro.com/shop/files/documents/46_020.pdf Material safety data sheet for 20x SSC buffer, Mediatech, Inc.]<br /> * [http://www.le.ac.uk/bl/phh4/probuffer.htm Molecular Cytogenetics and biology Buffers, University of Leicester]<br /> <br /> {{DEFAULTSORT:Ssc Buffer}}<br /> [[Category:Buffer solutions]]<br /> <br /> <br /> {{biochem-stub}}</div>

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