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Apparatus

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'''Moving-boundary electrophoresis''' ('''MBE''' also '''free-boundary electrophoresis''') is a technique for separation of chemical compounds by [[electrophoresis]] in a free [[Aqueous solution|solution]].<ref name="Marmur1982">{{cite journal|last1=Marmur|first1=Abraham|title=Moving-boundary electrophoresis: Theory and interpretation of "anomalies"|journal=Journal of Colloid and Interface Science|volume=85|issue=2|year=1982|pages=556–565|issn=0021-9797|doi=10.1016/0021-9797(82)90022-4|bibcode=1982JCIS...85..556M}}</ref><ref name="Stern1939">{{cite journal|last1=Stern|first1=Kurt G.|title=THE MOVING BOUNDARY METHOD FOR STUDYING Electrophoresis|journal=Annals of the New York Academy of Sciences|volume=39|issue=1|year=1939|pages=147–186|issn=0077-8923|doi=10.1111/j.1749-6632.1939.tb55374.x|bibcode=1939NYASA..39..147S|s2cid=85079793}}</ref>

==History==
Moving-boundary electrophoresis was developed by [[Arne Tiselius]] in 1930.<ref name="Tiselius1930">{{cite book|author=Arne Tiselius|title=The Moving Boundary Method of Studying the Electrophoresis of Proteins|url=https://books.google.com/books?id=SHsoSQAACAAJ|year=1930|publisher=Inaugural Dissertation Upsala}}</ref> Tiselius was awarded the 1948 Nobel Prize in chemistry for his work on the separation of [[colloid]]s through electrophoresis, the motion of charged particles through a stationary liquid under the influence of an electric field.

==Apparatus==
The moving-boundary electrophoresis apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any mixture of charged components such as a [[protein]] mixture. On applying voltage, the compounds will migrate to the anode or cathode depending on their charges. The change in the refractive index at the boundary of the separated compounds is detected using [[schlieren]] optics at both ends of the solution in the cell.<ref name="Westermeier2001">{{cite book|author=Reiner Westermeier|title=Electrophoresis in Practice|url=https://books.google.com/books?id=tai_QgAACAAJ|date=21 March 2001|publisher=Wiley|isbn=978-3-527-30300-7}}</ref>

==See also==
*[[Capillary electrophoresis]]

== References ==
{{reflist}}

== External links ==
*[https://web.archive.org/web/20011122103534/http://www.geocities.com/bioelectrochemistry/tiselius.htm Arne Wilhelm Kaurin Tiselius] — Information on Tiselius compiled from various sources.

{{electrophoresis}}

[[Category:Electromagnetism]]
[[Category:Electrophoresis]]
[[Category:Colloidal chemistry]]

Moving-boundary electrophoresis - Revision history

imported>Indeblues: /* Apparatus */