http://debianws.lexgopc.com/wiki143/index.php?action=history&feed=atom&title=Cleaved_amplified_polymorphic_sequence 2026-05-08T22:28:32Z Revision history for this page on the wiki MediaWiki 1.43.1 http://debianws.lexgopc.com/wiki143/index.php?title=Cleaved_amplified_polymorphic_sequence&diff=7240317&oldid=prev 2021-03-24T02:30:48Z

<p>The term is POLYacrylamide gel electrophoresis (PAGE)</p> <p><b>New page</b></p><div>{{no footnotes|date=November 2017}}<br /> The &#039;&#039;&#039;cleaved amplified polymorphic sequence&#039;&#039;&#039; (&#039;&#039;&#039;CAPS&#039;&#039;&#039;) method is a technique in [[molecular biology]] for the analysis of [[genetic marker]]s. It is an extension to the [[restriction fragment length polymorphism]] (RFLP) method, using [[polymerase chain reaction]] (PCR) to more quickly analyse the results.<br /> <br /> Like RFLP, CAPS works on the principle that genetic differences between individuals can create or abolish [[restriction endonuclease]] restriction sites, and that these differences can be detected in the resulting DNA fragment length after digestion. <br /> <br /> In the CAPS method, PCR amplification is directed across the altered restriction site, and the products digested with the restriction enzyme. When fractionated by [[agarose gel electrophoresis|agarose]] or [[polyacrylamide gel electrophoresis]], the digested PCR products will give readily distinguishable patterns of bands. Alternatively, the amplified segment can be analyzed by [[allele-specific oligonucleotide]] (ASO) probes, a process that can often be done by a simple [[dot blot]].<br /> <br /> ==See also==<br /> * [[Restriction fragment length polymorphism|RFLP]]<br /> <br /> ==References==<br /> {{Reflist}}<br /> <br /> ==External links==<br /> * https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechCAPS.shtml<br /> <br /> [[Category:DNA profiling techniques]]<br /> [[Category:Molecular biology]]<br /> <br /> <br /> {{molecular-cell-biology-stub}}</div>

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