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A '''cellular microarray''' (or cell microarray) is a [[laboratory]] tool that allows for the [[multiplex (assay)|multiplex]] interrogation of living [[cell (biology)|cells]] on the surface of a solid support. The support, sometimes called a "chip", is spotted with varying materials, such as [[antibodies]], [[proteins]], or [[lipids]], which can interact with the cells, leading to their capture on specific spots. Combinations of different materials can be spotted in a given area, allowing not only cellular capture, when a specific interaction exists, but also the triggering of a cellular response, change in [[phenotype]], or detection of a response from the cell, such as a specific [[secretion|secreted]] factor.

There are a large number of types of cellular microarrays:

# [[Reverse transfection]] cell microarrays. David M. Sabatini's laboratory developed reverse-transfection cell microarrays at the [[Whitehead Institute]], publishing their work in 2001.
# [[PMHC Cellular Microarrays]]. This type of microarray were developed by Daniel Chen, Yoav Soen, Dan Kraft, Patrick Brown and Mark Davis at [[Stanford University Medical Center]].

==References==
* Chen DS, Davis MM (2006) Molecular and functional analysis using live cell microarrays. Curr Opin Chem Biol 10:28-34
* Chen DS, Soen Y, Stuge TB, Lee PP, Weber JS, Brown PO, Davis MM (2005) Marked Differences in Human Melanoma Antigen-Specific T Cell Responsiveness after Vaccination Using a Functional Microarray. PLoS Med 2: 10: e265 ([https://archive.today/20130113063447/http://medicine.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pmed.0020265]) {{open access}}
* Soen Y., Chen D. S., Kraft D. L., Davis M. M. and Brown P.O. (2003) Detection and characterization of cellular immune responses using peptide-MHC microarrays. PLoS Biol. 1: E65 (http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0000065) {{open access}}
* Chen DS, Davis MM (2005) Cellular immunotherapy: Antigen recognition is just the beginning. Springer Semin Immunopathol 27:119–127
* Chen DS, Soen Y, Davis MM, Brown PO (2004) Functional and molecular profiling of heterogeneous tumor samples using a novel cellular microarray. J Clin Oncol 22:9507 (https://web.archive.org/web/20041020122342/http://meeting.jco.org/cgi/content/abstract/22/14_suppl/9507)
* Soen Y, Chen DS, Stuge TB, Weber JS, Lee PP, et al. (2004) A novel cellular microarray identifies functional deficiences in tumor-specific T cell responses. J Clin Oncol 22:2510
* Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Nature. 2001 May 3;411(6833):107-10. [https://www.ncbi.nlm.nih.gov/pubmed/11333987?dopt=Citation Pubmed link]

[[Category:Biotechnology]]

Cellular microarray - Revision history

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