View source for Dispase

'''Dispase''' is a [[protease]] which cleaves [[fibronectin]], [[collagen]] IV, and to a lesser extent collagen I. It is found in some [[bacteria]] and can be isolated from culture filtrates of ''[[Bacillus polymyxa]]''. It can be extracted, purified, and used in research. It can be particularly useful to separate embryonic [[epithelia]] and [[mesenchyme]]. Dispase II is specific for the cleavage of [[leucine]]-[[phenylalanine]] bonds.

Dispase is often used to digest adhering primary cells in culture, since this treatment turned out to be milder than [[trypsin]] digestion (Sinclair et al., 2013).

A recent article also finds that dispase can digest serine-phenylalanine.<ref>{{cite journal | author = Weimer |display-authors=et al | year = 2006 | title =  A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases| journal = Analytical Biochemistry | volume = 352 | issue = 1| pages = 110–119 | doi=10.1016/j.ab.2006.02.029| pmid = 16564490 }}</ref>

Dispase intravitreal injection can be used in the modeling of [[proliferative vitreoretinopathy]] in different animals.<ref>{{cite journal
 |vauthors=Erdiakov AK, Tikhonovich MV, Rzhavina EM, Gavrilova SA
 |pmid=26263683
 |title=THE CHARACTERISTICS OF RETINA AT THE DEVELOPMENT OF PROLIFERATIVE VITREORETINOPATHY IN RATS AFTER INTRAOCULAR INJECTION OF CONCANAVALIN A AND DISPASE
 |journal=Ross Fiziol Zh Im I M Sechenova
 |date=May 2015
 |volume=101
 |issue=5
 |pages=572–85
}}</ref><ref>{{cite journal
 |vauthors=Cantó Soler MV, Gallo JE, Dodds RA, Suburo AM
 |pmid=12457862
 |title=A mouse model of proliferative vitreoretinopathy induced by dispase
 |journal=Exp Eye Res
 |date=November 2002
 |volume=75
 |issue=5
 |pages=491–504
}}</ref><ref>{{Cite journal|last=Tikhonovich|first=Marina V.|last2=Erdiakov|first2=Aleksei K.|last3=Gavrilova|first3=Svetlana A.|date=2017-06-21|title=Nonsteroid anti-inflammatory therapy suppresses the development of proliferative vitreoretinopathy more effectively than a steroid one|journal=International Ophthalmology|volume=38|issue=4|language=en|pages=1365–1378|doi=10.1007/s10792-017-0594-3|pmid=28639085|issn=0165-5701}}</ref>

==References==
{{reflist}}

{{Proteases}}
{{Enzymes}}
{{Portal bar|Biology|border=no}}

[[Category:EC 3.4]]


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